QPS NEUROPHARMACOLOGY

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QPS Neuropharmacology is a preclinical full-service contract research organization (CRO) focusing on CNS diseases, Orphan diseases and mental disorders.

The availability of highly predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes makes QPS the first choice for most needs in CNS drug development.

Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal Dementia (FTD), Niemann-Pick Disease, Autism Spectrum Disorder (ASD), Schizophrenia, Lewy Body Dementia (LBD) and other neurodegenerative diseases.

QPS Neuropharmacology is a preclinical full-service contract research organization (CRO) focusing on CNS diseases, Orphan diseases and mental disorders.

The availability of highly predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes makes QPS the first choice for most needs in CNS drug development.

Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal Dementia (FTD), Niemann-Pick Disease, Autism Spectrum Disorder (ASD), Schizophrenia, Lewy Body Dementia (LBD) and other neurodegenerative diseases.

Why choose us?

Customer satisfaction is our absolute priority

Your timeline is our timeline

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Every study is custom-built

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Scientific input to study design and data interpretation

Extensive experience with virtually all drug targets and treatment types

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Wide range of validated models and techniques for comprehensive compound tests from a single source

AAALAC certification ensures highest quality standards

QPS Austria AAALAC accrediation Logo

QPS NEURO NEWS

R6/2 Mice - Late Stage Disease Model for Huntington‘s Disease Research

 CAA – independent risk factor for cognitive dysfunction of Alzheimer’s disease

QPS Austria is your partner for visualization and quantification of Cerebral Amyloid Angiopathy (CAA) in murine tissues. Here we evaluated CAA in APPSL and 5xFAD transgenic animals by measuring the overlap of 6E10 and collagen IV labeling in the isocortex and hippocampus. The strongest progressive increase of CAA signal could be measured in APPSL mice from 6 to 12 months. In 5xFAD mice the total CAA signal was already high at 6 month of age resulting in a weaker signal increase in later age groups. These data suggest, that both mouse models display a progressive and robust vascular CAA pathology, an independent risk factor for cognitive dysfunction of Alzheimer’s disease.

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Figure 1: Progression of CAA in different brain areas of APPSL and 5xFAD mice over age. A: Increase of CAA over age in the cerebral cortex and hippocampus measured in overlap with collagen IV labeling expressed as sum immunoreactive (IR) signal normalized to 12 month old transgenic animals. Two-way ANOVA with Bonferroni’s post hoc test, n = 6 per group, females only. *p<0.05; **p<0.01; ***p<0.001. ntg: non-transgenic animal. B: Representative images of amyloid by 6E10 labeling (green) on collagen IV (red) positive vessels (submeningeal arteries) in APPSL mice over age compared to a non-transgenic littermate (ntg). Nuclei are labeled with DAPI (blue).

Meet us at the AAIC 2019 conference in Los Angeles, California, USA

The QPS Austria Neuropharmacology team will attend the Alzheimer’s Association International Conference 2019 and are pleased to meet you at our booth # 212.

Additionally, our scientists are presenting our newest research results in four posters:

14th of July

P1-112: „PROGRESSIVE INCREASE OF ALZHEIMER’S DISEASE PATHOLOGY IN 5XFAD TRANSGENIC MICE” Tina Loeffler, Magdalena Temmel, Jörg Neddens, Irene Schilcher, Birgit Hutter-Paier.

16th of July

P3-113: “UNTANGLING ALZHEIMER´S DISEASE HALLMARKS IN SENSORY SYSTEMS OF RODENT MODELS” ” Joerg Neddens, Magdalena Temmel, Meritxell Aguilo Garcia, Tina Loeffler, Irati Aiestaran Zelaia, Vera Niederkofler, Stefanie Flunkert, Birgit Hutter-Paier.

17th of July

P4-061“TAU PHOSPHORYLATION PROFILE OF HTAU TRANSGENIC MICE” ” Joerg Neddens, Tina Loeffler, Magdalena Temmel, Irene Schilcher, David Amschl, Birgit Hutter-Paier

P4-062: “IN VITRO MODELS TO STUDY TAU AGGREGATION, PHOSPHORYLATION AND UPTAKE” ” Tina Loeffler, Irene Schilcher, Stefanie Flunkert, Birgit Hutter-Paier

Join us from July 14-18 in the Los Angeles Convention Center, California, USA

R6/2 Mice - Late Stage Disease Model for Huntington‘s Disease Research

 R6/2 Mice – Late Stage Disease Model for Huntington‘s Disease Research

R6/2 Mice express the N-terminal fragment of the human huntingtin (HTT) protein with ~120 CAG repeats under the control of the human HTT promoter. Animals are a highly used model for the development of drugs against Huntington’s disease.
Mice present the following phenotype:

  • 50 % survival at ~100 days
  • Learning deficits at 10 weeks
  • Motor deficits at 8 weeks
  • Strong HTT overexpression at 12 weeks
  • Strong neuroinflammation at 12 weeks

R6/2 have a mean survival of 15 weeks and show re-learning deficits at the age of 10 weeks (Figure 1).

Figure 1: Survival and Learning Deficits in R6/2 Mice. A: Survival curve of R6/2 mice and non-transgenic littermates (ntg); n = 8/group. B: Two choice swim test of 10 week old R6/2 mice and ntg littermates; n = 15/group; Two-way ANOVA with Bonferroni’s post hoc test. Mean ± SEM. *p<0.05; **p<0.01; ***p<0.001.

All performed motor tests revealed strong motor deficits of R6/2 mice already at 8 weeks of age (Figure 2).
Figure 2: Motor Deficits of R6/2 Mice A: Hanging time in seconds in the wire suspension test of 4 and 12 weeks old R6/2 and ntg littermates; B: Number of bites per episode in the pasta gnawing test; C: Latency to fall off the rod in the RotaRod test; D: Number of slips on a 10 mm square beam of the beam walk test. B-D: 4, 8 and 12 week old R6/2 and ntg littermates. A-D: n = 15/group; Two-way ANOVA with Bonferroni‘s post hoc test. Mean ± SEM. *p<0.05; **p<0.01; ***p<0.001.
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R6/2 mice show a highly increased huntingtin expression in the caudate putamen and hippocampus at the age of 12 weeks (Figure 3).
Figure 3: Quantification of Huntingtin in 12 Week old R6/2 Mice. A: Immunoreactive area of huntingtin in percent in the caudate putamen B: Immunoreactive area of huntingtin in percent in the caudate putamen. A and B: n = 4/group; Unpaired t-test. Mean + SEM. ***p<0.001.
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R6/2 mice show increased astrocytosis in the caudate putamen and hippocampus at the age of 12 weeks (Figure 4). Similar effects could be observed for activated microglia in the same brain regions (data not shown).

Figure 4: Quantification of Astrocytosis in 12 Week old R6/2 Mice. A: Immunoreactive area of GFAP in percent in the caudate putamen B: Immunoreactive area of GFAP in percent in the caudate putamen. A and B: n = 4/group; Unpaired t-test. Mean + SEM. ***p<0.001.

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Webinar “Predictive Disease Models for CNS Drug Development“

 

In November 2018, Birgit Hutter-Paier, PhD, the Director of Neuropharmacology at QPS Austria presented this webinar about validated transgenic and non-transgenic in vitro and in vivo models covering most targets of AD, PD, HD and other neurodegenerative diseases.

 

Birgit Hutter-Paier

  You will learn:

  • When to use animal models in your drug discovery program
  • How to choose the most appropriate animal model for your therapeutic or mechanism of action
  • How to design the most efficient “first-pass” proof-of-concept study
  • Best practices for submitting compounds and sample requirements for a successful study

 

Brought to you by ADDF ACCESS, a program of the ADDF and Science Exchange, as part of their commitment to connect researchers developing CNS drugs with technologies that can help accelerate breakthrough discoveries.

Format: 30 minutes with 10 minutes Q&A

Watch recording!

In Vitro Services

QPS Austria’s Neuropharmacology Department provides research services with numerous standardized cell culture systems including transgenic and non-transgenic cell lines, glial cells, primary chicken and rat peripheral and central nervous system neurons of different developmental stages and organotypic brain slices. New models are developed and validated on request.

In Vivo Services

We have more than 15 years experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects. Several customized behavioral tests, including motor, cognitive, and emotional assays, are offered to phenotype mouse and rat models and to evaluate effects of compounds in different in vivo models.

Ex Vivo Services

Our ex vivo services cover a full range of histological services, a biobank composed of various specimen derived from our in-house in vivo and in vitro models, and numerous well established tests for biomarkers. We are happy to test new protocols and establish new markers to meet your specific needs.