Mitochondria Related Assays
Cell lines and primary neurons can be exposed to different toxins and examined for mitochondrial activity and
mitochondrial membrane depolarization.
Mitochondrial Activity (MitoTracker)
Figure: Effect of FCCP on active mitochondria in primary hippocampal rat neurons. FCCP treatment significantly reduced MitoTracker Red labeling in somata and neurites of primary rat hippocampal neurons (see graph). Images indicate (left) control neurons and (right) neurons upon FCCP treatment. MitoTracker labeling is indicated in red, MAP2 labeling in green.
Remark: This assay can also be performed with other mitochondria related toxins.
Mitochondrial Membrane Depolarization (JC-1)
Figure: Effect of ionomycin and MPP+ on mitochondrial membrane depolarization by JC-1 labeling. Images indicate control cells and cells lesioned with ionomycin or MPP+ for 3 hours. In intact mitochondria, JC-1 forms red fluorescent aggregates, whereas green fluorescent monomers indicate mitochondria with depolarized membrane potential.
Remark: These assays can also be customized for primary neurons.