Induced Disease Models

As a leading CRO for CNS drug development, QPS Austria provides induced animal models of several neurodegenerative and rare diseases. Animal models that recapitulate some pathophysiological and behavioral characteristics of human disorders can be used to develop new disease-specific drugs, screen compounds for lesion efficacy or toxicity, or study gene function. Furthermore, QPS Austria offers virus injections of different types to induce a plethora of different pathologies by introducing cDNA or shRNA of your target protein.

Virus Injections

Tau Seeding

Biosafety level 2 experiments are becoming more and more indispensable in the field of neurodegenerative and rare disease research. With this method, target proteins can be expressed or suppressed in distinct brain regions by intracerebral injection or more systemically by intraventricular or even intravenous injection of virus expressing cDNA or shRNA of the target protein.
The fully AAALAC accredited animal facility of QPS Austria maintains a Biosafety Level 2 (BSL2) laboratory for mouse and rat experiments, including but not limited to e.g. Lenti-, Adeno- and Adeno-associated (AAV) virus injections.
To analyze the effect of specific viruses for the induction or treatment of Alzheimer’s or other cognitive diseases, injections in the mouse ERC can be performed.
In the here presented seeding study, P301L tau AAV was injected in the ERC of APPSL mice and spreading of tau in the hippocampus could be observed.

Figure 1: Recombinant Adeno-associated virus serotype 9 (AAV9) with human P301L Tau gene or empty vector was intracerebrally injected into the ERC of 3 months old male APPSL mice. A: Verification of injection site by ink injection into the entorhinal cortex. B: Tau expression in the ERC after P301L Tau virus injection. C: Spreading of tau into the hippocampus after P301L tau injection into the ERC of APPSL mice. All analyses were performed 1 month after virus injection.

Alzheimer’s Disease

Scopolamine Treated Mice

Scopolamine is a tropane alkaloid drug with competitive antagonism at muscarinic acetylcholine receptors (mAChR). Systemic application of scopolamine disrupts the performance on several reference memory tasks, such as object discrimination, radial arm maze, water maze and fear conditioning. The scopolamine-induced cognitive impairment can be reversed by cholinesterase inhibitors. Therefore this model can be used to mimic cognitive dysfunction observed in dementia and AD and is a useful initial screening method to identify therapeutic candidates.

Parkinson’s Disease

MPTP Mouse Model

Mice that receive acute, chronic or subchronic administration of the pyridine toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) selectively lose significant numbers of dopaminergic neurons in two midbrain structures, the substantia nigra (SN) and the ventral tegmental area (VTA). Loss of dopamine cells in the SN mimics the clinical condition of Parkinson’s disease and leads to motor dysfunction. The dopaminergic loss in mouse VTA is of unknown relevance to Parkinson’s disease but may contribute to the cognitive deficits of Parkinson’s disease because of these neurons’ projections to the frontal cortex.
MPTP treated mice are a suitable model to study motor deficits and the loss of dopaminergic neurons as well as possible influences of drugs on these parameters.

Figure 1: Open Field test. Animals were injected with 4x 20 mg/kg MPTP or vehicle at one day. Two days after treatment, animals were tested in the open field test for activity. n= 9-10 per group; unpaired t-test; Mean + SEM. ***p<0.001.
Figure 2. Tyrosine hydroxylase (TH) quantification in the substantia nigra after MPTP lesion. Animals were injected with 4x 20 mg/kg MPTP or vehicle at one day. Six days after treatment, animals were sacrificed and the substantia nigra analyzed for TH levels. n = 3-5 per group; unpaired t-test; Mean + SEM. *p<0.05.
6-OHDA Lesion Model

Unilateral local application of 6-hydroxydopamine (6-OHDA), a neurotoxic substance that preferentially affects catecholaminergic neurons, is a well-established model for analyzing effects of loss of dopaminergic neurons in the substantia nigra pars compacta and their major target area, the dorsal striatum.
The advantage of unilateral lesions is twofold, I. the contralateral hemisphere serves as an internal control that facilitates comparison of lesion effects between individuals and experimental groups during histological analysis, and II. lesion efficacy in individual animals can be estimated prior to sacrifice using certain behavioral tests.

Figure 1. Coronal sections of 6-OHDA-injected mice. Tyrosine hydroxylase (TH) immunofluorescent labeling (upper picture). GFAP and TH immunofluorescent labeling to visualize astrocytosis (lower picture). Cpu: Caudate-Putamen; VTA: ventral tegmental area; SNpc: substantia nigra pars compacta. Arrows indicate injection canal.
Figure 2: Analysis of mice in the corridor test revealed that 6-OHDA injected mice preferred the right corridor side over the left while sham injected mice had no preference. n = 16-30; Mean + SEM. Two way ANOVA with Bonferroni’s post hoc test. ***p<0.001.

 

Haloperidol Induced Catalepsy

Catalepsy is a neuronal condition that can be observed in Parkinson’s disease, epilepsy, catatonia but also as adverse reaction to prescribed medications e.g. against schizophrenia. It is characterized by seizures with a loss of sensation and consciousness accompanied by rigidity of the body. By treating rats with the dopamine D2 receptor antagonist haloperidol, catalepsy can be acutely induced for several hours, mimicking the typical symptoms like loss of consciousness and rigidity.
Sprague Dawley rats were subcutaneously injected with 1mg/kg Haloperidol. After 30 minutes, catalepsy was measured and additionally, animals showing a full catalepsy were treated orally with the positive compound or vehicle. Animals were retested for catalepsy at several time points after drug administration.
Catalepsy was evaluated by gently placing the front limbs of each rat over an 8 cm high horizontal bar; catalepsy was measured as time animals spent motionless. A cut-off time of 120 seconds was used.

Figure 1: Haloperidol-induced Catalepsy after treatment with a positive compound: Time animals spent rigid after s.c. treatment with 1 mg/kg haloperidol, followed by oral treatment with a positive compound. Animals were repeatedly tested for catalepsy. n = 10. Mean ± SEM. Two-way ANOVA with Bonferroni’s repeated measure post hoc test. ***p<0.001. T.I.: Test item / positive compound.

 

Gaucher Disease

Conduritol-B-Epoxide (CBE)-Induced Gaucher Disease

Gaucher disease (GD) is the most common lysosomal storage disorder. The main pathological hallmark is the intracellular accumulation of glucosylceramide and glucosphingosine as a result of reduced glucocerebrosidase (GCase) enzyme activity due to mutations in the β-glucocerebrosidase gene (GBA). Conduritol-beta-epoxide (CBE) is a specific inhibitor of GCase activity and can thus be used to induce Gaucher disease in vivo.
C57Bl/6 mice were intraperitoneally injected with 100 mg/kg CBE on 9 consecutive days and brains analyzed for neuroinflammation.

Figure 1. Cortical astrocytosis and activated microglia of CBE-treated mice. GFAP (A) and CD11b (B) immunoreactive (IR) area in percent. n = 9 per group; unpaired t-test; Mean + SEM. ***p<0.001. Representative images of cortical tissue of vehicle-treated or CBE-treated mice. Note the occurrence of extremely enlarged microglia (arrowhead) and increased GFAP in CBE-treated mice.

 

Schizophrenia

MK-801-Induced Schizophrenia

Non competitive NMDA receptor antagonists, such as MK-801, are shown to produce complex symptoms that mimic positive and negative symptoms, as well as the cognitive deficits of schizophrenia. MK-801 (hydrogen maleate) impairs learning and memory functions that depend on the hippocampus and the amygdala. MK801 also produces various effects on rodent behavior including deficits in sensory processing, hyperactivity, stereotypy and ataxia. Antipsychotic drugs, such as clozapine, used in the treatment of schizophrenia have been shown to improve MK-801 induced cognitive impairment in mice.
C57Bl/6 mice were injected with 0.2 mg/kg MK-801 and immediately analyzed.

Figure 1: Open field test. A: Distance traversed of MK-801-treated animals compared to sham treated control (CTRL) B: Hyperactivity level of MK-801-treated animals compared to sham treated control. Mean ± SEM; Two-way ANOVA with Bonferroni’s post hoc test. *p<0.05; **p<0.01; ***p<0.001.

 

Amphetamine (AMPH) or Phencyclidine (PCP) Treated Rat Model

The most widely validated animal models of the positive, negative and cognitive symptoms of schizophrenia involve administration of the dopamine-releasing drug, d-amphetamine in combination with the benzodiazepine Chlordiazepoxide (AMPH) or an open channel NMDA receptor blocker, phencyclidine (PCP).  Pretreatment with Clozapine (CZP) can reverse the observed effects.

  • AMPH increases activity in the Open Field test 10 minutes after treatment
  • AMPH effect can be decreased by CZP


Figure 2: Open field behavior of amphetamine (AMPH)-treated Sprague Dawley rats. A: Distance traversed in cm/5min over time; Two-way repeated measurements ANOVA with Bonferroni‘s post hoc test. Mean ± SEM. B: distance traversed in cm/5min, 20 minutes after start of the analyses; One-way ANOVA with Bonferroni‘s post hoc test. Mean + SEM. CTRL = Control; CTRL, AMPH, AMPH+CZP: n = 8-10 per group;. *p<0.05; **p<0.01.

 

  • PCP increases the startle response of Sprague Dawley rats in the prepulse inhibition test, effect can be prevented by CZP
  • PCP decreases prepulse inhibition of Sprague Dawley rats in the prepulse inhibition test, effect can be reversed by CZP, effect of PCP depends on prepulse intensity


Figure 1: Startle response and Prepulse inhibition of PCP-treated Sprague Dawley rats. A: Startle amplitude in gram of PCP, PCP+CZP or CZP treated animals at 120 dB; One-way ANOVA followed by Bonferroni‘s post hoc test. B: Prepulse inhibition in percent of vehicle-, PCP- or PCP+CZP-treated animals using 4 different dB intensities; Two-way ANOVA with Bonferroni‘s post hoc test. A,B: n = 10; Mean + SEM; **p<0.01;***p<0.001.

Neuroinflammation

Lipopolysaccharide (LPS) Induced Neuroinflammation

Neuroinflammation is a common feature of different neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, Frontotemporal dementia, Amyotrophic Lateral Sclerosis and many more. Recent research suggests that targeting neuroinflammation might present a valid method to treat neurodegenerative diseases. To model neuroinflammation independent from other disease relevant pathologies, mice can be peripherally injected with lipopolysaccharide (LPS). Several different LPS treatment regimes are published. Here we present exemplarily the effect of four 0.5mg/kg LPS injection on consecutive days.
Other treatment protocols can be performed according to your needs.

Figure 1: Immunofluorescent labeling of the medial hippocampus of LPS treated mice. Animals were intraperitoneally injected with 0.5mg/kg LPS (A) or vehicle (B) on 4 consecutive days. Tissue was labeled with CD68 antibody (red) for macrophages, IBA1 antibody (green) for microglia and DAPI (blue) for nuclear staining.

 

Multiple Sclerosis

Cuprizone Induced Multiple Sclerosis

Cuprizone is a copper chelator, that causes rapid demyelination and gliosis, and rapid proliferation of glia subtypes. The cuprizone mouse model captures several aspects of Multiple Sclerosis (MS) pathology like demyelination / remyelination, cognitive decline, altered activity and motor deficits. The cuprizone model is the most frequently used model among the toxin-induced MS models and is used to study mechanisms of oligodendrocyte turnover, gliosis as well as motor capabilities. This animal model is thus suitable to assess certain aspects of the MS pathology and to test pharmaceutical compounds.

C57Bl/6 mice were fed with 0.3 % cuprizone chow for 1 month. Behavioral changes were analyzed within the last week of cuprizone treatment.

Figure 1: Beam walk test, MAO activity and astrocytosis of C57BL/6 mice after 4 weeks of cuprizone treatment. A: latency to traverse a 10 mm wide square beam in seconds. B: MAO activity in brain lysates. C: Quantification of astrocytosis in the hippocampus by GFAP labeling. Mean + SEM; n = 10 per group; unpaired t-test/Mann Whitney test; ***p<0.001.

 

QPS Austria offers a custom tailored study design for these models and we are flexible to accommodate to your special interests. We are also happy to advice you and propose study designs. Induced models have a explicitly low turn-around time by using wild type mice of commercial breeding companies.
We would be glad to test your compounds in these mouse models!

As with all other in vivo models we are also ready to provide samples (brain tissue, CSF etc.) from these animals for analyses in your laboratory.

We are happy to receive your inquiry.