Lateral Lesion Tests

Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models.

New behavioral tests are continuously developed and validated.

Corner Turn Test

The Corner Turn Test measures sensorimotor impairments by unilateral damage to the dopaminergic system.

The apparatus consists of two similar walls that are connected in a pointed angle of 30° with a small opening at the tip of the angle so the animal’s curiosity encourages entrance into the corner. Once the animal enters the corner the attempts of turns towards the impaired or non-impaired body side are recorded. Performance of test is videotaped and the number of turns is manually counted.

corner turn test figureCorner Turn Test of 6-OHDA lesioned mice. Sham lesion on top (orange, n=16), 6-OHDA lesion on bottom (blue, n=30). Symbols show individual animals, vertical lines are mean ± SEM. T-test p = 0.0664.

Corridor Test
corridor test img1

Adapted from Graelish et al. 2010; PMID: 20529122.

The Corridor Test is a sensitive system to measure sensorimotor impairment by unilateral damage to the dopaminergic system.

The apparatus consists of a long narrow corridor with 10 pairs of flanking jars. Each jar contains a sugar pellet. Animals are starved overnight and habituated to the corridor and the sugar pellets. After habituation, sugar pellets are placed in the jars that are positioned at 5cm intervals along the corridor always two jars next to each other. The animal is put in one corner of the corridor and videotaped while eating sugar pellets from the jars. The first 20 nose pokes are manually counted and the proportion between right and left jar are calculated.

 

corridor test figureNumber of nose pokes per side in the Corridor Test after a 6-OHDA lesion of one hemisphere. The dotted line represents number of evenly distributed nose pokes. Mean + SEM; Sham: n = 16; 6-OHDA lesion: n = 26. One Way ANOVA: ***p<0.001.

Rotation Test

The Rotation Test is a sensitive system to measure sensorimotor impairment by unilateral damage to the dopaminergic system.

The rotometer bowls are hemispheres with a 50 cm diameter. In this apparatus unilaterally lesioned animals are more likely to show rotation behavior compared to e.g. the open field apparatus. Animals are placed in the rotometer bowls and their behavior during the test session is videotaped. The test is performed under standard light illumination.

Behavioral parameters like clockwise/counterclockwise rotations are automatically counted with a tracking system.