Cytokines constitute a significant portion of the immuno- and neuromodulatory messengers that can be released by activated microglia. QPS Neuropharmacology provides several cell culture systems including the microglial cell line BV-2, primary mouse microglia or organotypic brain slices (hippocampus or whole brain). All of these are suitable for testing the anti-inflammatory effects of your developmental compounds.
Murine microglial BV-2 cells are stimulated with LPS for 24h in the presence or absence of a test item (e.g. Ibudilast). The inflammatory response is determined by the release of various cytokines in the supernatant or/and in cell extracts using multiplex cytokine arrays. A huge panel of inflammatory mediators can be detected among these IFN-gamma, IL-1beta, IL-6, IL-10, KC, TNF-alpha.
Figure 1: Immuno-modulatory effects of Ibudilast on various cytokines in LPS stimulated BV-2 cells.
Figure 2: Cytokine detection of organotypic hippocampal brain slices upon LPS stimulation.