BACHD transgenic Rat Model
BACHD rats overexpress full-length human mutant HTT (mHTT) with 97 alternating CAA/CAG repeats on a bacterial artificial chromosome (BAC).
- mHTT aggregates
- Motor deficits
- Reversal learning deficits
- Reduced anxiety
- Striatal alterations
Figure 1: RotaRod and Morris water maze. A: RotaRod test was performed from month 1 to 5. Latency to fall off the rod in seconds. B: 7 months old BACHD rats present with a significantly longer latency to find the platform in the reversal trials on day 9 of testing, animals present no learning deficits during the first 8 days of training (data not shown). Two-way ANOVA followed by Bonferroni posthoc test. Mean ± SEM. N = 15 per group. **p<0.01 ***p<0.001.
- Homozygous BACHD rats present the same phenotype as heterozygous BACHD rats but with an earlier disease onset
Figure 2. Passive Avoidance and Barnes Maze test of 2 and 5 months old homozygous BACHD rats, respectively. A: Latency to enter the dark chamber on day 2. B: Latency to enter the target hole during learning and relearning in the Barnes Maze test; C: Latency to enter the target hole during the probe trial (memory) during the Barnes Maze test. Mean ± SEM; A,C: Mann Whitney U-test; B: Two way ANOVA followed by Bonferroni‘s posthoc test. n=16-20 per group; **p<0.01.