TDP-43 transgenic Mouse Model
All those phenotypical changes are accompanied by severe neurodegeneration seen in the brain, especially in thalamic neurons of TAR6/6 mice. Most central thalamic nuclei suffer from a severe break down of dendritic network, which is followed by neuronal loss and a strong local neuroinflammation. The latter as seen by astro- and microgliosis can also be found in the hypothalamus, the medulla oblongata and to a lower extent in spinal cord grey matter.
Hence these mice offer a unique opportunity to counteract TDP-43 related neurodegeneration in a fast and severe motor and memory phenotype model.
Figure 2: Latency to fall from the RotaRod in 6 weeks (A) and 14 to 17 weeks old TAR6/6 transgenic mice (B) compared to non-transgenic littermates (ntg). A: tg N = 5; ntg N = 7; B: tg N = 11-3; ntg N = 16-5. Unpaired t-test or Mann Whitney test depending on normal distribution. Data are shown as mean±SEM. *p<0.05; ***p<0.001.
Figure 3: Time spent in the open arms of the EPM of 6 (A) and 18 weeks (B) old TAR6/6 transgenic mice compared to non-transgenic littermates (ntg). A: tg N = 5; ntg N = 6; B: tg N = 8; ntg N = 16. Data are shown as mean ±SEM. Data were analyzed by Mann Whitney test. ***p<0.001.
We would be happy to test your compounds in this mouse model! The most common readouts are:
• Motor behavior (RotaRod, Beam Walk, Wire Hanging, Nest Building)
• Memory (CFC)
• TARDBP expression, pTDP-43
• Dendritic network break down and neuronal loss
• Gliosis (astro- and activated microglia)
• Looking for something else? Please contact us!
QPS Austria offers alternative models allowing the performance of the same type of studies also in every other commercially available mouse line, e.g. SOD1G93A(HI)
You might be also interested in these related topics
• TDP in APP transgenic mouse model
As with all other in vivo models we are also ready to provide samples (brain tissue, CSF etc.) from these animals for analyses in your laboratory.
We are happy to receive your inquiry.
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