New behavioral tests are continuously developed and validated.
Barnes Maze Test
The test consists of a circular arena with equally distributed holes on its periphery with one hole serving as target zone that is equipped with the animal’s home cage. The animal can use proximal or distal visual cues to locate and remember the target zone. Automated recordings of consecutive trials provide details on the rodent’s navigation in the arena and thus delivers information about their learning and memory performance.
Experimental set-up of the Barnes Maze test. Learning and reversal of hippocampal learning protocol. The test can also be performed with a striatal learning protocol (not shown).
Contextual Fear Conditioning Test
Animals are trained and tested on 2 consecutive days. On the training day, animals are placed in a chamber and receiving a conditioned stimuli / unconditioned stimuli package (tone/foot shock). Contextual memory is tested 24 hours after training. The animal is placed into the same chamber as during the training and freezing behavior is automatically recorded. Cued memory is scored one hour after the contextual test in a novel, modified chamber where the animal receives the auditory cued stimulus and freezing behavior is again automatically recorded.
The time spent freezing during the contextual test and tone-dependent test are defined as index of fear-related learning and memory. Cued and contextual fear conditioning is a task that measures the ability of the animal to learn and remember an association between an aversive experience and environmental cues. All parameters of the test are measured automatically with a tracking system.
Fear conditioning of 4-, 6-, 9- and 12- month old APPSL transgenic mice compared to non-transgenic littermates. Freezing time in percent of the whole test combined. Mean + SEM. 4m APP n=16, WT n=17; 6m APP n=19, WT n=21; 9m APP n=21, WT n=19; 12m APP n=13, WT n=22; Statistical analyses: Two-way-ANOVA with Bonferroni post-test compared to WT. **p<0.01; ***p<0.001.
Morris Water Maze Test
Assessment of spatial learning in the Morris water maze. Learning curves showing escape latencies during 4 testing days of 6-, 9- and 12-month old animals. 6-month old APPSL n=19, WT n=21; 9-month old APPSL n=21, WT n=19; 12-month old APPSL n=13, WT n=22; mean ± SEM. Two-way-ANOVA with Bonferroni’s post-test compared to non-transgenic (ntg) littermates. *p<0.05; **p<0.01.
Novel Object Recognition Test
Novel Object Recognition Test in Scopolamine treated female Sprague Dawley rats at the age of 10 weeks. Animals were treated with 2.5mg/kg Scopolamine. Mean + SEM. n = 7 per group. One-Way ANOVA; *p<0.05.
Passive and Active Avoidance Test
In this task, the animals learn to refrain from stepping through a door to an apparently safer but previously punished dark chamber. A light and a dark chamber are connected in an apparatus by a guillotine door.
Acquisition: The mouse is placed into the illuminated chamber of the apparatus upon entering the dark chamber, the door will close and an electrical stimulus is applied. Retention test: 24 hours after acquisition, the animal is placed back into the illuminated chamber again. The time until the animal visits the dark chamber (latency) is measured and serves as an index of emotional memory. All parameters are measured using an automated tracking system.
Passive Avoidance Test in male Scopolamine treated Sprague Dawley rats at the age of 10 weeks. Animals were treated once with 2.5mg/kg Scopolamine. Scopolamine: n = 12, Vehicle: n = 8; mean+SEM. T-test; **p<0.001.
Two Choice Swim Test
A corridor-like tank is filled with tainted water and illuminated on one end. The hidden platform is positioned either at the dark or lit end of the tank. The mouse is placed in the centre of the tank. During acquisition of the task, each animal performs several training trials by swimming towards or away from the light to find the platform. Following the acquisition phase, the platform is switched towards the opposite end of the corridor, while the light source remains at the former position to test for reversal learning. In all trials, both choice and latency to reach the platform are recorded.
Figure 1. Two Choice Swim Test of 12-week old R6/2 mice. Shown is the number of wrong choices compared to non-transgenic mice. Mean ± SEM. n = 14 per group. Two Way ANOVA; **p<0.01.
Figure 2. Two Choice Swim Test of 12-week old R6/2 mice. Shown is the escape latency compared to non-transgenic mice. Mean ± SEM. n = 14 per group. Two Way ANOVA; **p<0.01.
Spontaneous Alternation test
Testing occurs in a Y-shaped maze with three plastic arms oriented in a 120° angle. After introduction to the center of the maze, the animal is allowed to freely explore the three arms, where it should show a tendency to enter a less recently visited arm. This test is used to quantify cognitive deficits in transgenic animals and evaluate novel compounds for their effects on cognition.
Y-Maze Novelty and Memory
To test whether rodents prefer to spend time in new or familiar areas, one arm of the Y-maze is blocked and the animal is allowed to explore the other two arms. The rodent is then placed in the start arm and the blocked arm is opened, so the animal should show a tendency to enter the formerly blocked arm more frequently. The Y-maze test is particularly useful as an initial test of memory function in mice.
Y-Maze, longitudinal evaluation of 9-month old 5xFAD transgenic and non-transgenic littermates. Graphs represent the longitudinal evaluation of the alternation rate (left graph) and number of arm entries of testing 1 and 2 in the Y-Maze of 5xFAD Tg and nTg mice; n=8 per group, mixed gender. Mean ± SEM. Two-Way ANOVA. **p<0.01.