Besides transgenic rodent models QPS Neuropharmacology provides one of the most frequently used animal models for PD utilizing unilateral injection of 6- hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB) in rats, which results in total denervation of the dopaminergic nigrostriatal pathway.
Figure 1: Unilateral injection into the MFB of saline (A, B) or 6-OHDA (C, D) in male Wistar Han rats. Immunofluorescent labeling on coronal brain sections revealed an almost complete loss of tyrosine hydroxylase (TH; orange) in the ipsilateral caudate putamen (CPu; C as well as in the substantia nigra (SN; D) after 6-OHDA injection. Saline injection did not affect TH immunoreactivity (A, B). Cell nuclei were labeled with DAPI (blue). Arrows in A and C indicate CPu, arrows in B and D indicate SN.
Figure 2: Cylinder test of 6-OHDA lesioned rats. Ipsilateral fore limb use after unilateral 6-OHDA injection into the MFB. 6-OHDA significantly increased the frequency of ipsilateral fore limb use compared to saline injected control animals 3, 4 and 5 weeks after surgery. Number in columns gives group size (n); mean + SEM; mixed effects analysis with Bonferroni´s post hoc test; ***p<0.001.
Figure 3: Amphetamine-induced ipsilateral rotation of 6-OHDA-injected rat MFB. Rotational response to D-amphetamine 3 weeks after 6-OHDA injection. 2.5 mg/kg D-amphetamine was injected i.p. on the test day. 30 minutes later, rotations were analyzed in the rotometer bowl. 6-OHDA-injected rats performed almost only ipsilateral rotations while saline-injected rats performed approximately the same number of ipsi- and contralateral rotations (A). The total number of ipsilateral rotations during a 60 min period was significantly increased in 6-OHDA-injected rats compared to saline-injected control rats (B); Number in columns gives group size (n); mean + SEM, Mann Whitney test: ***p<0.001.
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Hepatic and neuronal phenotype of NPC1−/− mice. Estibaliz Santiago-Mujica, Stefanie Flunkert, Roland Rabl, Joerg Neddens, Tina Loeffler, Birgit Hutter-Paier; Heliyon. 2019 Mar 14;5(3):e01293. doi: 10.1016/j.heliyon.2019.e01293
Correction: Further investigation of phenotypes and confounding factors of progressive ratio performance and feeding behavior in the BACHD rat model of Huntington disease. Clemensson EKH, Clemensson LE, Fabry B, Flunkert S, Riess O, Wronski R, Nguyen HP. PLoS One. 2019 Mar 7;14(3):e0213755. doi: 10.1371/journal.pone.0213755. eCollection 2019.
Metabolic disease and ABHD6 alter the circulating bis (monoacylglycerol) phosphate profile in mice and humans. Grabner GF, Fawzy N, Pribasnig M, Trieb M, Taschler U, Holzer M, Schweiger M, Wolinski H, Kolb D, Horvath A, Breinbauer R, Ruelicke T, Rabl R, Lass A, Stadlbauer V, Hutter-Paier B, Stauber RE, Fickert P, Zechner R, Marsche G, Eichmann TO, Zimmermann R. J Lipid Res. 2019 Mar 20. pii: jlr.M093351. doi: 10.1194/jlr.M093351.