MEET QPS AT AD/PD™ 2024; March 5-9, Lisbon, Portugal

신경 가소성

Neuronal Plasticity can be defined as the ability of the nervous system to change structure, function and organization of neurons in response to new experiences, emerging from changes of developmental and environmental situations, as well as other factors (e.g. injuries) affecting the conditions of the nervous system or the whole organism. Neuronal plasticity such as NeurogenesisSynaptogenesis and Neurite Outgrowth once thought to occur mainly during development has now been recognized as key features of adult brain function.

The balance between neurogenesis and cell death plays a critical role not only in the embryonic brain development but also in the maintenance of the adult brain. Alterations in these processes are seen in many neurodegenerative diseases. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage at early stages of Alzheimer’s disease (AD). Emerging evidence has indicated that compromised neurogenesis in the adult hippocampus represents an early critical event in the course of AD. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on neurogenesis of hippocampal neurons.

Neurogenesis Solution

Primary rat hippocampal neurons are treated with compounds on DIV1 for a total of 48 h. After 24 h, BrdU [10 µM] is added. Compounds are present throughout the assay. On DIV3, after 48h incubation cells are subjected to indirect immunofluorescence analysis. Digital images from primary rat hippocampal neurons are analyzed for the percentage of BrdU positive cells compared to the total number of neurons (NeuN positive cells) using a software-supported automatic quantification method. Analysis is performed using Image Pro Plus software. Approximately 10000 to 15000 cells per group are screened for BrdU positive nuclei.

Neurogenesis

Figure: Effects of Fibroblast Growth Factor (FGF) on neurogenesis of primary rat hippocampal neurons.

Synapse formation but especially the synaptic maintenance (synaptogenesis) is critical for neuronal health and disease. Loss of synaptic maintenance is not only restricted to AD. Defects in synaptic maintenance most likely also contribute to the synaptic vulnerability in other neurodegenerative disorders as Huntington’s, Parkinson’s or Niemann Pick disease. A therapeutic strategy that favors synaptic maintenance may be a beneficial target for many neurodegenerative diseases. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on synaptogenesis primary hippocampal neurons.

Synaptogenesis Solution

To evaluate effects of Test Item (a commercially available CDK inhibitor) and Reference Item (RI) on synaptogenesis in primary rat hippocampal neurons, cells are treated on DIV6 for 24h. Cells are subjected to immunofluorescence analysis using synapsin and MAP2. Nine systematic uniform random frames are imaged per coverslip at 63x magnification as 10 image deep z-stacks on an AxioImager.Z1 microscope equipped with LED illumination and a Axio MRm B&W camera with 1x opto-coupler. The MAP2 image served to delineate the cells. The area of interest is limited to the neurites of the cells. Results are shown as % immunoreactive area of synapsin co-localization to MAP2 area.

Synaptogenesis

Figure: Effects of Test and Reference Item (RI) on synaptogenesis in primary rat hippocampal neurons.

During development, neurons become assembled into functional networks by growing out axons and dendrites (collectively neurite outgrowth) that connect synaptically to other neurons. Strikingly, neurons retain their capacity for growth and synapse formation in the adult brain. Only those neurons that manage to establish adequate contacts with neighbor cells escape cell death. To stimulate and accelerate neurite outgrowth is thus important for restoring neuronal function after injury or from neurodegenerative diseases. A promising strategy for drugs targeted against neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease is promoting the regeneration of neurons. Thus, new therapies are focusing on identifying new molecules that affect neurite outgrowth. For this purpose, QPS Austria provides cell culture models to assess the neurotrophic effects of your developmental compounds.

Neurite Outgrowth Solution 1

Primary rat (mouse) hippocampal or cortex neurons are treated with compounds for 48 h on DIV1. Cells are subjected to indirect immunofluorescence analyis using anti-beta-III tubulin antibody. The following parameters using image based analysis (Axio.Imager Z1 microscope (20x), 10 image deep z-stacks, collapsed to 2D (ImageProPlus), 12 images per slide from 12 predefined fields, Image enhancement, filtering) are determined of approximately 200 cells per experimental group.

  • number of neurites
  • total length of neurites
  • length of the longest neurite
Neurite Outgrowth

Figure: Effects of Compound X and reference item (RI) on neurite outgrowth of primary rat hippocampal neurons.

Neurite Outgrowth Solution 2

Adult rat dorsal root ganglion (DRG) explants are cultured under serum free conditions in the presence or abesence of neurotrophic factors for 5 days. Explants are are subjected to indirect immunofluorescence analyis using anti-beta-III tubulin antibody. Parameters such as the neuritic halo (the total area specifically occupied by neurites) are analyzed by using a software-supported automatic quantification method.

Adult dorsal root ganglia

Figure 2: Neuritic halo of adult dorsal root ganglion explants upon treatment with NGF.

요청사항이 있는 경우 John Yi 한국 에이전트([email protected])에게 한국어로 연락하십시오.

정보 요청

동물 모델

QPS Neuropharmacology는 형질전환 질병 모델을 생성하고, 특화하고, 유지하는 데 풍부한 경험이 있으며 20년이 넘도록 이를 신약 실험 프로젝트에 사용했습니다.
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조직학 서비스

QPS Neuropharmacology는 조직 표본 채취부터 실험 절차 및 결과가 모두 포함된 연구 보고서 제출에 이르는 전체 조직학 서비스를 제공합니다. 당사의 접근법은 고객의 특정 요구 사항에 따라 어떤 서비스든 맞춤화할 수 있는 일련의 절차적 구성 요소에 기반합니다. 고객은 당사의 워크플로우에서 시작점과 종점을 자유롭게 선택할 수 있습니다.
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바이오 마커

QPS Neuropharmacology는 다양한 질병 분석을 위한 분자 생물학적 방법에 있어 많은 선택지를 제공합니다. 해당 유닛은 QPS Neuropharmacology에서 수행하는 체외체내 연구를 지원합니다. 그리고 동일한 기술을 사용해 외부 전임상 연구 또는 임상 연구의 표본 분석을 정기적으로 수행합니다. 임상 표본뿐 아니라 전임상 체외 및 체내 연구에 동일한 분석 방법을 사용함으로써 고객 연구 프로젝트의 해석적 가치를 증가시킵니다.
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