Neuroinflammation is a growing field of research in the fight against neurodegenerative diseases and other disorders affecting the central nervous system. We have already shown that 4L/PS-NA mice exhibit strong inflammation in visceral organs and cortical brain regions (Schiffer et al., 2020). Since the cerebellum of 4L/PS-NA mice presents with a highly disturbed morphology (Schiffer …
Injection of 6-OHDA in the rat MFB
Besides transgenic rodent models QPS Neuropharmacology provides one of the most frequently used animal models for PD utilizing unilateral injection of 6- hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB) in rats, which results in total denervation of the dopaminergic nigrostriatal pathway. Figure 1: Unilateral injection into the MFB of saline (A, B) or 6-OHDA …
Inflammasome activation in organotypic brain slices
LPS-stimulated organotypic hippocampal slices provide an optimal tool to investigate the impact of developmental compounds on inflammasome activation in vitro. By maintaining the three-dimensional structure and interplay of different cell types of the postnatal brain, this system closely resembles the in vivo situation while offering several advantages for early screenings: take supernatant samples anytime test …
Neurofilament light chain – biomarker of high translational value!
Biomarkers and their use in neurodegenerative disease research delineates a rising area of research and may provide an important link between preclinical disease models and evaluation of disease progression in patients. Neurofilament light chain (NF-L), a neuronal cytoplasmic protein, is increased in a variety of neuronal diseases due to axonal damage. A strong increase of …
Plaque-associated inflammation
Interested in plaque-associated inflammation? QPS Neuropharmacology is your partner for visualization and quantification of plaque-associated inflammation. Figure 1 shows an exemplary quantification process to evaluate microglia (Iba1 labeled) adjacent to amyloid-beta plaques (6E10 labeled). Multichannel immunofluorescence (up to four different antibodies plus DAPI labeling in one experiment), whole-slide imaging, and quantitative image analysis, allows detailed …
