In vitro model to study A53T α-synuclein in PD
Different preformed recombinant human α-synuclein (α-syn) fibrils were evaluated for their toxicity and seeding properties on primary cortical neurons in vitro. Our results show that monomeric human α-syn has no impact on cell viability, while preformed wild type and A53T human α-syn fibrils have toxic effects on primary cortical neurons. This toxic effect was exceeded …
TSPO as sensitive biomarker for neuroinflammation in the Huntingon’s disease mouse model R6/2
Neuroinflammation is a growing field of research in the fight against neurodegenerative diseases and other disorders affecting the central nervous system. TSPO is an outer mitochondrial membrane protein, that has recently raised some interest for research in Huntington’s disease since its upregulation has been associated with the activation of glia cells. By labelling GFAP, Iba1, …
Neuroinflammation in the Niemann-Pick disease mouse model NPC1-/-
Neuroinflammation is a growing field of research in the fight against neurodegenerative diseases and other disorders affecting the central nervous system. We have previously shown that NPC1-/- mice exhibit strong inflammation (Santiago-Mujica et al., 2019). To further elucidate inflammation in the brain of NPC1-/- mice, we now evaluated CD45 analysis in the thalamus (A) and …
Neuroinflammation in the Gaucher disease mouse model 4L/PS-NA
Neuroinflammation is a growing field of research in the fight against neurodegenerative diseases and other disorders affecting the central nervous system. We have already shown that 4L/PS-NA mice exhibit strong inflammation in visceral organs and cortical brain regions (Schiffer et al., 2020). Since the cerebellum of 4L/PS-NA mice presents with a highly disturbed morphology (Schiffer …
Injection of 6-OHDA in the rat MFB
Besides transgenic rodent models QPS Neuropharmacology provides one of the most frequently used animal models for PD utilizing unilateral injection of 6- hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB) in rats, which results in total denervation of the dopaminergic nigrostriatal pathway. Figure 1: Unilateral injection into the MFB of saline (A, B) or 6-OHDA …