Interested in plaque-associated inflammation?
QPS Neuropharmacology is your partner for visualization and quantification of plaque-associated inflammation. Figure 1 shows an exemplary quantification process to evaluate microglia (Iba1 labeled) adjacent to amyloid-beta plaques (6E10 labeled).
Multichannel immunofluorescence (up to four different antibodies plus DAPI labeling in one experiment), whole-slide imaging, and quantitative image analysis, allows detailed evaluation of pathological changes.
The image analysis is macro-based using Image-Pro 10 software, enabling fast and cost-efficient quantification of large batches of images. Importantly, the results are operator-independent and fully reproducible.
Figure 1: Immunolabeling of 10 month old 5xFAD mouse brain section and the analysis process to quantify plaque-associated microglial activation. A: Merged signal of amyloid-beta (6E10 in red) and microglia (Iba1 in white); cell nuclei are labeled with DAPI (blue). B-D: Single channel images of 6E10. Scale bar: 50 µm. (B), Iba1 (C), and DAPI (D). E-I: Quantification process: First, 6E10-immunoreactive positive objects (plaques) are identified by adequate thresholding and morphological filtering (E). Second, mask images of the evaluated 6E10-positive area are created (F) and the borders of 6E10-positive objects are extended by 15 µm in all directions (G, H). Finally, Iba1-positive objects (activated microglia) are quantified in the vicinity of amyloid-beta plaques (I).
Meet us at the AAIC 2019 conference in Los Angeles, California, USA
The QPS Austria Neuropharmacology team will attend the Alzheimer’s Association International Conference 2019 and are pleased to meet you at our booth # 212.
Additionally, our scientists are presenting our newest research results in four posters:
14th of July
P1-112: „PROGRESSIVE INCREASE OF ALZHEIMER’S DISEASE PATHOLOGY IN 5XFAD TRANSGENIC MICE” Tina Loeffler, Magdalena Temmel, Jörg Neddens, Irene Schilcher, Birgit Hutter-Paier.
16th of July
P3-113: “UNTANGLING ALZHEIMER´S DISEASE HALLMARKS IN SENSORY SYSTEMS OF RODENT MODELS” ” Joerg Neddens, Magdalena Temmel, Meritxell Aguilo Garcia, Tina Loeffler, Irati Aiestaran Zelaia, Vera Niederkofler, Stefanie Flunkert, Birgit Hutter-Paier.
17th of July
P4-061“TAU PHOSPHORYLATION PROFILE OF HTAU TRANSGENIC MICE” ” Joerg Neddens, Tina Loeffler, Magdalena Temmel, Irene Schilcher, David Amschl, Birgit Hutter-Paier
P4-062: “IN VITRO MODELS TO STUDY TAU AGGREGATION, PHOSPHORYLATION AND UPTAKE” ” Tina Loeffler, Irene Schilcher, Stefanie Flunkert, Birgit Hutter-Paier
Join us from July 14-18 in the Los Angeles Convention Center, California, USA