QPS Neuropharmacology offers a broad selection of molecular biological methods for the analysis of various diseases. The unit supports in vitro and in vivo studies performed at QPS Neuropharmacology; routine sample analyses from external preclinical or clinical studies are additionally performed using the same techniques. Using the same analysis methods for preclinical as well as clinical samples increases the translational value of your research project.
Specifics of the readouts depend on the indication. Please see the respective in vitro and in vivo models for the most relevant readout of each model.
For example, our main readout for Alzheimer’s disease is the analyses of different Aβ species. We routinely analyze brain and CSF samples for Aβ 38/40/42 and tau/ptau levels from animal models as well as from humans.
As highly translational marker of neurodegeneration, neurofilament light chain measurement can be performed in body fluids (CSF, plasma, serum) of neurodegenerative disease animal models or cell culture supernatants.
Furthermore, customized assays can be set up and validated, cloning and transfection of cell lines can be performed.
- Tissues
- Cerebrospinal fluid (CSF)
- Blood, plasma, serum
- Urine
- Cell extracts & supernatants
- In vitro protein aggregation
- Brain tissue e.g. processed to soluble or insoluble fractions
- Extraction and purification of DNA/RNA/protein
Protein quantification and enzyme activity assessment, with colorimetric, fluorescence or luminescence detection can be performed:
- Western blotting
- WES
- ELISA
- Enzymatic assays (e.g. Monoamine Oxidase, Acetyl/Butylcholinesterase activity)
We also offer protein analysis with all kits from the MSD platform (Mesoscale Discovery). By using this multiplex electrochemiluminescence immunosorbent assay technique, minimal sample volumes can be used to obtain several readouts from one analysis.
- e.g. for various inflammation marker/cytokines (TNF-a, IFN-γ, various interleukines,…)
- Aβ
- Tau
- α-synuclein and many more
Additionally, also oxidative stress and lipid metabolism can be analyzed in house:
- Colorimetric assays such as TBARS assay for oxidative stress
- Assessment of total cholesterol levels in plasma and tissues
If you are interested in gene expression and mRNA analysis, we can also evaluate your samples with:
- Quantitative real-time RT-PCR/gene expression analysis for any gene of interest
- Standard RT-PCR for any gene of interest
Furthermore, customized assays can be set up and validated, cloning and transfection of cell lines can be performed.
Statistical Data Analysis
- Professional statistical data analysis (unbiased and representative) performed by our team of scientists and biostatisticians
- Conscientious quality checks of measurements and results
Reporting of Experiments and Results
- At the end of each study our customers receive a full study report including all experimental procedures, qualitative and quantitative assessment of the results, as well a comprehensive statistical analysis
- Constant updates throughout the study are provided if desired