Primary microglial cultures enable you to study the effect of your compound specifically on microglia, which are prepared from early postnatal mouse pups (see Figure).
In addition, we recently set up a method to isolate microglial cells from adult mice by Magnetic Cell Sorting (MACS). Generation of a pure microglial fraction from adult brains of transgenic or even compound-treated mice opens a variety of new possibilities to assess the efficacy of your treatments.
Cytokine release by mouse primary microglia after LPS stimulation for 12 h. Data are displayed as aligned dot blots with group means (n=6 per group). Mean ± SEM. One-way ANOVA followed by Bonferroni`s multiple comparison post hoc test compared to LPS group. **p<0.01, ***p<0.001.