The balance between neurogenesis and cell death plays a critical role not only in the embryonic brain development but also in the maintenance of the adult brain. Alterations in these processes are seen in many neurodegenerative diseases. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage at early stages of Alzheimer’s disease (AD). Emerging evidence has indicated that compromised neurogenesis in the adult hippocampus represents an early critical event in the course of AD. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on neurogenesis of hippocampal neurons.
Primary rat hippocampal neurons are treated with compounds on DIV1 for a total of 48 h. After 24 h, BrdU [10 µM] is added. Compounds are present throughout the assay. On DIV3, after 48h incubation cells are subjected to indirect immunofluorescence analysis. Digital images from primary rat hippocampal neurons are analyzed for the percentage of BrdU positive cells compared to the total number of neurons (NeuN positive cells) using a software-supported automatic quantification method. Analysis is performed using Image Pro Plus software. Approximately 10000 to 15000 cells per group are screened for BrdU positive nuclei.
Figure: Effects of Fibroblast Growth Factor (FGF) on neurogenesis of primary rat hippocampal neurons.