Synapse formation but especially the synaptic maintenance (synaptogenesis) is critical for neuronal health and disease. Loss of synaptic maintenance is not only restricted to AD. Defects in synaptic maintenance most likely also contribute to the synaptic vulnerability in other neurodegenerative disorders as Huntington’s, Parkinson’s or Niemann Pick disease. A therapeutic strategy that favors synaptic maintenance may be a beneficial target for many neurodegenerative diseases. For this purpose, QPS Neuropharmacology provides a cell culture model to assess effects of your developmental compound on synaptogenesis primary hippocampal neurons.
To evaluate effects of Test Item (a commercially available CDK inhibitor) and Reference Item (RI) on synaptogenesis in primary rat hippocampal neurons, cells are treated on DIV6 for 24h. Cells are subjected to immunofluorescence analysis using synapsin and MAP2. Nine systematic uniform random frames are imaged per coverslip at 63x magnification as 10 image deep z-stacks on an AxioImager.Z1 microscope equipped with LED illumination and a Axio MRm B&W camera with 1x opto-coupler. The MAP2 image served to delineate the cells. The area of interest is limited to the neurites of the cells. Results are shown as % immunoreactive area of synapsin co-localization to MAP2 area.
Figure: Effects of Test and Reference Item (RI) on synaptogenesis in primary rat hippocampal neurons.