zQ175DN-KI mice (JAX# 029928) have the mouse Htt exon 1 replaced by the human Htt exon 1 sequence with ~180-220 CAG repeats. Furthermore, the neo cassette of these KI mice was deleted (DN). zQ175DN KI mice show:
- High mHTT levels
- Reduced body weight
- Reduced general health
- Reduced motor skills
Figure 1: Quantification of mHTT by sandwich immunosorbent assay using the MesoScale Discovery platform in the isocortex of 5.5 months old mixed sex zQ175DN KI and WT mice. WT: n = 3; zQ175DN KI: n = 12. Mean + SEM. Unpaired t-test. ***p<0.001.
Figure 2: Beam Walk test of 5.5. months old mixed sex zQ175DN KI and WT mice. Number of slips on a 10 mm square beam. n = 12 per group. Mean + SEM; unpaired t-test. **p<0.01.