hTau mice express human tau derived from a human PAC, H1 haplotype, known as 8c mice, while murine tau is knocked out by a targeted disruption of exon 1. Animals express all 6 human tau isoforms and present with accumulations of phosphorylated tau, aggregated tau and PHFs.
Figure 1: Quantitative analysis of ptau Ser202 (A) and Ser396 (B) level in the cortex of 3 and 12 month old hTau mice compared to non-transgenic littermates. Immunoreactive area in percent. n = 8 per group. Mean + SEM; Two-way ANOVA with Bonferroni’s post hoc test; ***p<0.001. Similar results were observed for hippocampal ptau Ser202 and ptau Ser396.
Soluble total and phosphorylated tau levels in the brain of hTau mice. Soluble tissue fractions of 3, 6, 9, and 12 month old animals were analyzed for total, pThr231 and pThr181 levels by MSD immunosorbent assay. One-way ANOVA with Newman’s Keuls multiple comparison test. Mean ± SEM; n = 2-4.