The glucose tolerance test is a standard procedure to test for insulin resistance. Specifically, the uptake of glucose from the blood by cells is regulated by insulin. An impairment of the uptake speed indicates problems with the maintenance of glucose homeostasis (e.g. diabetes, obesity and cardiovascular diseases).
To measure glucose tolerance, mice are fasted overnight before blood is collected for measuring baseline blood glucose concentrations. Subsequently, a single dose of a 20% glucose solution is applied intraperitoneally prior to additional blood glucose concentration measurements at 15, 30, 60 and 120 min post injection.
Assessment of glucose tolerance in C57BL/6 mice treated with high fat diet for 12 weeks. Blood glucose level measurements per group measured at baseline (A), after 6 weeks (B) and 12 weeks (C) of high fat/control diet. Mean ± SEM. n = 10 per group. Two-way ANOVA with Bonferroni´s post hoc test. *p<0.05; ***p<0.001.