The Open Field Test is used to test rodents for activity and anxiety.
A Plexiglas Open Field (TSE-System®) is used to test the activity of rodents. Infrared photo beams detect movements on the ground but also rearing (standing on the hind limbs). Testing is performed under standard room lighting conditions in naïve animals. The number of fecal boli is counted as measure of emotionality. A tracking system automatically measures the activity, hyperactivity, rearing, thigmotaxis, and distance traversed.
Open field behavior of MK-801 treated C57Bl/6 mice. A: Distance traversed of MK801-treated animals compared to sham-treated controls. MK801-treated mice show increased locomotion starting 20 minutes after treatment. B: Hyperactivity level of MK801-treated animals compared to sham-treated controls. MK801-treated mice show increased hyperactivity levels starting 20 minutes after treatment. n = 10. Mean ± SEM. Two-way ANOVA with Bonferroni’s post hoc test. *p<0.05; p<0.001.