The wire suspension test detects neuromuscular abnormalities of motor strength. For this test, a wire cage lid is used where duct tape is placed around the perimeter to prevent the mouse from walking off the edge. The animal is placed on the top of the cage lid. The lid is slightly shaken three times to force the mouse to grip the wires. Afterwards, the lid is turned upside down. The lid is held at a height of approximately 50 – 60 cm above a soft underlay, high enough to prevent the mouse from jumping down, but not high enough to cause harm in the event of a fall. The latency to fall down is quantified. A 90 or 300 second cut-off time is used depending on the model.
Wire hanging test in 1, 2, 3 and 6 month old Line 61 mice compared to non-transgenic littermates. Latency to fall off the wire cage lid. tg: n = 13 – 15, ntg: n = 11 – 15; Mean + SEM; Two-way ANOVA with Bonferroni’s post hoc test. *p<0.05; ***p<0.001.