Grooming is a normal behavior of healthy mice to clean themselves. It can be distinguished between social grooming (grooming of another mouse) and autogrooming (self grooming). In socially disturbed animals, autogrooming can be strongly increased. This behavior can mostly be observed when animals are housed separately.
For this test, animals are therefore housed separately for three days. On the 4th day, animals are placed in the open field box and after a habituation phase of 10 minutes, animals are filmed for another 10 minutes. It is measured, how much time animals spent autogrooming within this 10 minutes.