Cyclodextrin is an Effective Reference Compound for NPC1-/- Mouse Studies
The strong pathology of NPC1-/- mice, a Niemann-Pick type 1 mouse model, can be significantly reduced by cyclodextrin treatment. Here we show that cyclodextrin (2-hydroxypropyl-ß-cyclodextrin, HPBCD improves cholesterol levels as well as liver enzyme levels. Subcutaneous treatment of NPC1-/- mice once weekly for 3 weeks thus reduces liver cholesterol levels (Fig.1A) while increasing plasma triglyceride …
Nitroglycerin- Induced Migraine Mouse Model
Injecting wild type mice with glyceryl trinitrate (a.k.a. nitroglycerin, NTG) is a fast and robust method to induce a migraine-like symptomatology that can efficiently be treated with an available migraine medication. Migraine, and its associated headache and neurological symptoms, is a severely impairing condition affecting more than a billion people worldwide. Although migraine is one …
New AAV2 hA53T-α-syn-induced PD mouse model
Injecting wild type mice unilaterally with AAV2 hA53T-α-syn virus particles is a fast and at the same time solid method to model Parkinson’s disease brain pathologies. A single unilateral injection of AAV2 hA53T-α-syn into the substantia nigra causes selective expression of hA53T-α-syn in the substantia nigra as well as the caudate putamen of the ipsilateral …
Tau Seeding for Modeling Tauopathies
Injection of purified tau aggregates from tauopathy patients into the mouse brain can recapitulate main pathological features of the patients tauopathy. This approach thus allows to analyze tauopathy-specific pathologies with a high translational value. For the injection, 9-10 weeks old hTau mice expressing human tau but lacking endogenous tau were used. Mice were unilaterally injected …
Ultrasonic Vocalization Measurements in Fmr1-KO mice
Measurement of ultrasonic vocalizations (USV) provides a unique and gentle method to study social behavior in rodents. Mice emit ultrasonic vocalizations in different social contexts throughout their lifespan, including pups when separated from the dam and adult males when exposed to a sexually receptive female. In the latter context, male mice typically emit ultrasonic vocalizations …