Neurodegenerative disorders, including multiple sclerosis (MS), Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), amyotrophic lateral sclerosis (ALS), tauopathies, and age-related macular degeneration (ARMD), are associated with chronic neuroinflammation and elevated levels of several cytokines.
Chronic neuroinflammation includes not only long-standing activation of microglia and subsequent sustained release of inflammatory mediators, but also the resulting increased oxidative and nitrosative stress.
Stimulation of in vitro cultures with LPS or other pro-inflammatory agents results in increased cytokine release into the supernatant. We are able to monitor this effect over time in the same cultures by single or multiplex MSD analyses. Up to 10 cytokines can be measured simultaneously from one sample. Create your own favorite combination from currently 38 available cytokines.
At QPS Neuropharmacology, currently 3 in vitro models for neuroinflammation based on LPS stimulation are established: